Glutamate - induced calcium signals stimulate CO production 1 in piglet astrocytes
نویسندگان
چکیده
Qi Xi, Dilyara Tcheranova, Shyamali Basuroy, Helena Parfenova, 4 Jonathan H. Jaggar, Charles W. Leffler 5 6 Laboratory for Research in Neonatal Physiology, Department of Physiology, University 7 of Tennessee Health Science Center, Memphis, TN 38163, USA 8 9 10 Running title: Calcium signaling in CO production by newborn pig astrocytes 11 12 13 Corresponding Author: 14 Dr. Charles W. Leffler 15 Department of Physiology 16 University of Tennessee Health Science Center 17 Memphis, TN 38163 18 USA 19 Tel.: 901-448-7122 20 Fax: 901-448-7126 21 Email: [email protected] 22 23 Articles in PresS. Am J Physiol Heart Circ Physiol (May 13, 2011). doi:10.1152/ajpheart.01277.2010
منابع مشابه
Glutamate-induced calcium signals stimulate CO production in piglet astrocytes.
Glutamate-stimulated, astrocyte-derived carbon monoxide (CO) causes cerebral arteriole dilation by activating smooth muscle cell large-conductance Ca(2+)-activated K(+) channels. Here, we examined the hypothesis that glutamate activates heme oxygenase (HO)-2 and CO production via the intracellular Ca(2+) concentration ([Ca(2+)](i))/Ca(2+)-calmodulin signaling pathway in newborn pig astrocytes. ...
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Astrocyte signals can modulate arteriolar tone, contributing to regulation of cerebral blood flow, but specific intercellular communication mechanisms are unclear. Here we used isolated cerebral arteriole myocytes, astrocytes, and brain slices to investigate whether carbon monoxide (CO) generated by the enzyme heme oxygenase (HO) acts as an astrocyte-to-myocyte gasotransmitter in the brain. Glu...
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Astrocytes can act as intermediaries between neurons and cerebral arterioles to regulate vascular tone in response to neuronal activity. Release of glutamate from presynaptic neurons increases blood flow to match metabolic demands. CO is a gasotransmitter that can be related to neural function and blood flow regulation in the brain. The present study addresses the hypothesis that glutamatergic ...
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Glutamate is the principal cerebral excitatory neurotransmitter and dilates cerebral arterioles to match blood flow to neural activity. Arterial contractility is regulated by local and global Ca(2+) signals that occur in smooth muscle cells, but modulation of these signals by glutamate is poorly understood. Here, using high-speed confocal imaging, we measured the Ca(2+) signals that occur in ar...
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Dilation of piglet pial arterioles to glutamate involves carbon monoxide (CO) produced from heme by heme oxygenase-2 (HO-2). Piglet cerebral microvessels and endothelial and smooth muscle cells grown on microcarrier beads were used to address the hypothesis that glutamate increases endothelial CO production by increasing HO-2 catalytic activity. CO was measured by gas chromatography/mass spectr...
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